Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEEFANRINLNTDLSNISKIICNKYNLGEYISDAVITVGYEDFNYTLETTQGKYCVKVFNKERTDIECEKYIERIELASKLNINTPKLYRVDEKSECNIKLENIKYRLCVFDYIDGKSFYDLGKIPNQDEINEIIRQMAIIHKATLQSDFIYD--KWA-IINFVKEFQN----KKQ-----------YLN----GN---DYKKLEELYNRFLQV------DIEKLPKAFVHGDIISTNVMKDKNNKLWIIDFAVSNYVPRIIDLAVSSCNLCLDTENKENTKKKVKMILEEYQKYNKLTDYELEQFPLFFDIANAMGILQISHLNTLGELSEEDKFWYDESEKGLEFSNEEFWKDIYVKK 2Q83 Chain:A ((19-307)) --------------LTELAENVLQGWDVQ-AEKIDVIQ----ALVWKVHTDSGAVCLKRIHRPE--KKALFSIFAQDYLAKKGMNVPGILPNKKGSLYSK-HG--SFLFVVYDWIEGRPFELT----VKQDLEFIMKGLADFHTASVGYQPPNGVPIFTKLGRWPNHYTKRCKQMETWKLMAEAEKEDPFSQLYLQEIDGFIEDGLRIKDRLLQSTYVPWTEQLKKSPNLCHQDYGTGNTLLGENEQIWVIDLDTVSFDLPIRDLRKMIIPLLDTTG--VWDDETFNVMLNAYESRAPLTEEQKQVMFIDMLFPY----------------------------------------------

General information: TITO was launched using: RESULT: Template: 2Q83.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 978 -47332 -48.40 -186.35 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -48.40 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.333

(partial model without unconserved sides chains): PDB file : Tito_2Q83.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2q83-query.scw PDB file : Tito_Scwrl_2Q83.pdb: