Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNLDGLLSR--IQ-AKTFSLVTHRDGATVYKAQTTQ-GYVFVKMYTRK---VDHERYCEILDELRA--FSLVPKLLNQFEW-ENYFV----VVMSALEGQPVNNILKNCSRTEKLSLLKEAGASLGSFHRAISPSRLFEMKFWKGRDDS-SKDPTLWNEHLKLMTTKWMSRIDPTKADYKEFSYELSELALYCKD-LREPTQFSLLHCDYIGRNLLATSC-NKISGVLDYEAARIGDAVYDLAKIVWLDMEFSDLELRHAFLQGWEKT-YG-EVVPQREFLTYVGIQCLAAIAWTDKHAPIEGNTIFRASAIKALKIVANEL 5IGI Chain:A ((12-297)) ---LYALAARHGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWV---VPQDSEVFAESFATALAALHAVPISAAV--------DAGMLIRTPTQARQKVADDVDR----VRREFV--VN-DKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLID-NTERVSGMIDWSEARVDDPAIDMAAH-LMVF---GEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSG----NEEYLAAAKAQL-------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1229 1450 1.18 5.45 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 1.18 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.333

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: