Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------KCNISILKDGKYFTQ--KQEIAGWTVKFHDLD----GNYVGKCKNAH--TYACTYVTCEDLAPGYSPG 1CS6 Chain:A ((294-382)) QPDWLDVITDTEADIGSDLRWSCVASGKPRPAVRWLRDGQPLASQNRIEVSGGELRFSKLVLEDSGMYQCVAENKHGTVYASAELTVQ--A------

General information: TITO was launched using: RESULT: Template: 1CS6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 88 -5866 -66.65 -112.80 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -66.65 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.536

(partial model without unconserved sides chains): PDB file : Tito_1CS6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1cs6-query.scw PDB file : Tito_Scwrl_1CS6.pdb: