Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKQKRKVPEVTEKKNKKLKKASAEGPLLGPEAAPSGEGAGSKGEAVLRPGLDAEPELSPEEQRVLERKLKKERKKE-ERQRLREAGLVAQHPPARRSGAELALDYLCRWAQKHKNWRFQKTRQTWLLLHMYDSDKVPDEHFSTLLAYLEGLQGRARELTVQKAEALMRELDEEG----SDPPLPGRAQRIRQVLQLLS--- 1XO5 Chain:A ((4-183)) LLAEYQDLTFLTKQEILLAHRRFCELLPQEQRSVESSLRAQVPFEQILSL---PELKANPFKERICRVFSTSPAKDSLSFEDFLDLLSVFSDTA-------------------------TPDIKSHYAFRIFDFDDDGTLNREDLSRLVNCLTRLSASEMKQLIDNILEESDIDRDGTINLSEFQHVISRSPDFASSFKIVL

General information: TITO was launched using: RESULT: Template: 1XO5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 613 22387 36.52 134.86 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 36.52 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.230

(partial model without unconserved sides chains): PDB file : Tito_1XO5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1xo5-query.scw PDB file : Tito_Scwrl_1XO5.pdb: