Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDAMTQGNFVY-----AFLKSCFTRLRGFAQFLL--PFLRPK---KRRGINY--YNPVELNKYAMQTLQKALKDDPGADIRDCLPLVYNSAMRVISKTKARKEEELKRPPTPEFRTKINYVENAEIIFPLSDEVVSLLLQYSDKDGE-LVGKQLVISLKQLIWESPKIWESAVRGVVLKCSDNVALKVIRQHPDYTEYTTLQYLAEKLPEIPVPSTYGVIRFKPFTAYFMSYIPSMTLAEAWPSLNHEGKTSVQEQLEEIFSKLRTLRKDDGYPLGGVGGEGVKEARVGNSAQEKTINTAASFVDLQFSLSEFLSKSYIQFIRTLQPPPPTGSVFTHGDVRKDNIMVDIGDNNTCTVTAIIDW-EDAGFYPDYFE--CTTLTRTWLPHVEDDWYNYLPPCIDPARFPHHWLIDRLWGIHHPIF 5EZ7 Chain:A ((2-392)) MPQALSTDILIVGGGIAGLWLNARLRRAGYATVLVESASLGGGQSVKSQGIIHGGTGASEAIADMPRRWRACLGSDGELDLRG-VRLLSEAHYLWSPVEQAKGEDLPPALRDKGFKGKAYRLT--EIVF----DVPDLIRRLAELAGDSLLAGERIEPLRE---------GRELAGLCVDGREIRAQRVVLSAGAGNEALLRELGLEQPAMQRRPLHMVMVKAATLKPLYAHCLGA----------GPKPRITVTTHPTR-----------DGQSVWYLGGDIAETDGVARDEAAQIAEARRELAKLLPWID--LGQAQWATLRVDRAEPAQSNLL-----RPDNAFLAEQGR------LLVGWPTKLALAPDFADRVCARLEEDGIRPSEHA---------ALPQLPRPPLAEPAWEVAFA--

General information: TITO was launched using: RESULT: Template: 5EZ7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1967 12281 6.24 35.39 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 6.24 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.169

(partial model without unconserved sides chains): PDB file : Tito_5EZ7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ez7-query.scw PDB file : Tito_Scwrl_5EZ7.pdb: