Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDQFLMSQRPGA---PVSPPAPRSVRSATPTISPQPQPQPQPQPQPQPPARPGSLATALRQALAAP-DDERGERISQLGLRPLTGGLNNYAFRWGT-PSEGEVCVKAYPSGDRWRAEREWRALSLLARRSVPWAPRPLWWDPHGSPPIVIMTMLPGREVRH-LDSPRERIAALDGLVDTLRAITAVPLEAGFETLVRVESIQFCLETLSDTWPRRLARVADSTLSADLRHLLRRWSNSGD-PEILGRP---APRVFSRGDGNLRNWLWDGAVCRCVD--FEYSGWSDRVFDAADLVEHPYREEIDDALWPGVIDALGITDADRPRFDAARRACAMRWA-AV-LWEKRGRQLGEFLVQLDRVRLLTRDL------ 4FWV Chain:A ((3-354)) AREALELAIRGGFHAYLVGPPSLGKHEALLAYLSTQSVETP-----------PDLLYVPLSERKVAVMTLPSGQ---EIHLAEAVEGLLLEVNRLDELFRQGSFLREKTQLEARFKEAREQQLEALRREAQEAGFAL-------GPVPAELSARLEEVTLGSMAASAELEVALRRLRRDWALHYLNNRFEPLFQRFPQARAY---LEALRARLARYAET-GEPLDPAQWRPNLLTSSSSGTPPPIVYEPYATAPRLFGRLDYLVDRGVWSTNVSLIRPGAVHRAQGGYLILDALSLKREGTWEAFKRALRNGQVEPV-----TEPQAPAGLEVEPFPIQMQVMLVGT-----PEAFEGLEEDPAFSELFRIRAEF

General information: TITO was launched using: RESULT: Template: 4FWV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1289 56211 43.61 175.66 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 43.61 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.156

(partial model without unconserved sides chains): PDB file : Tito_4FWV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4fwv-query.scw PDB file : Tito_Scwrl_4FWV.pdb: