Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTKTSHFSPLDFPEQLRTYIEGATLSDSSSHSGATVLYLDS---GYYLKIDQ---KRRLERETAIARWFETKGLG-TPVIDYLST-DKDYLLTKEAPGKDALAFLD--QPETICRTMAHALKKLHNLHPHNFPSENHLQTYKDRALKNYEKGEFYAKALLPQFQINSREEAFQLIQEQGHLLKTDAFIHGDACLPNFILKDA-SRFSCFIDLGLADFSDRHIDLFWAVWSLNYNL---DNPKYAELFLDYYGREE---VDTNKLRLVAAFEAFG 4H05 Chain:A ((17-266)) ------------------------WVVVEDGASGAGVYRLRGGGRELFVKVAALGAGVGLLGEAERLVWLAEVGIPVPRVVEGGGDERVAWLVTEAVPGRPASARWPREQRLDVAVALAGLARSLHALDWERCPFDRSLAVTVPQAARAVAEGSVDLEDLDEERKGWSGERLLAELERTRPADEDLAVCHGDLCPDNVLLDPRTCEVTGLIDVGRVGRADRHSDLALVLRELAHEEDPWFGPECSAAFLREYGRGWDGAVSEEKLAFYRLLDEF-

General information: TITO was launched using: RESULT: Template: 4H05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1067 18460 17.30 79.23 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 17.30 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.459

(partial model without unconserved sides chains): PDB file : Tito_4H05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4h05-query.scw PDB file : Tito_Scwrl_4H05.pdb: