Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTGAGAADATEDAATDATRGTDATRGTDATGGTDGPVPAARTAEEDRLVARVEELVGG-------RVTAIERQ--PRWRKAWYLTVDRD-----GAELALYVRGDKQIDAEPYPGLDREAAILRILERNGVPVPHVHGMSSDPIGIIMDRVPGTRDVAEAADDAQRRGIAEQYMEILARMHSIDVAEFAAAGIEVPTTPEGAQLAFVDANE-RL---YRRTKKAPEPLVEWALRWARRRLPTAGNRARFIHGDTG--QFLFVDG------RITCVYDFEASHIGDPLSDLAGLRTRAGTE---------------PL-GADIEHMIRHYQRVAGTT--------------IDPSALSFYTATYMLTAVMALSGPLTELRPADQQAEYLTWDLMVRRAMLWAIAEVEGVKIEPAPPVTLPTGYPARVTTVLEGTVRRMVPATGVDEANQSAALALAQWAGAMVAVGMANVERDLDRAAVLLGHRPADQAAADAELERFVLAAGPEHDLALLEYFAAQTEARVAEAVPMRTRLEQYALPKVVL 5FTG Chain:A ((5-361)) ----------------------------------------EPRTRRRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG-------EAMVLESVMFAILAERSLG-PKLYGIFPQ--GRLEQFIPSRRLDTEE---LSLPDISAEIAEKMATFHGMKMPFNKEPKW-LFGTMEKYL-KEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDY----------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1047 -371 -0.35 -1.34 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -0.35 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.264

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ftg-query.scw PDB file : Tito_Scwrl_5FTG.pdb: