Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLPDAWRRTFADAVIEQQTIGESRADVWRISPADSAA-LFVKCEQVMTWGELPGEVQRLRWLETLDLPAPRVLETTTEAEHNWLLMTAVPGRDLASSEQLSP---LQIVTLTAQALRELHAVAIDSCPFDHCLDKKIPLAREHVRAGLIDEEDFDDARLGRSAEDVFQQLLAERPPHEELVVTHGDACLPNLLAEHGRFSGFVDCGRLGVADRYQDLALAAHSITDNLGEQWLPAFFEAYGV-QPDQRRIAFYQLLDEFF 4FEV Chain:C ((31-272)) -----------------RDNVGQSGATIYRLYGKPNAPELFLKHGKGSVANDVTDEMVRLNWLTAF-MPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGE-FSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF

General information: TITO was launched using: RESULT: Template: 4FEV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1058 -23514 -22.22 -99.22 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain C : 0.77 3D Compatibility (PKB) : -22.22 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.434

(partial model without unconserved sides chains): PDB file : Tito_4FEV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4fev-query.scw PDB file : Tito_Scwrl_4FEV.pdb: