Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNRNKQKSKDYQSFLKNRHDKLNTSDDVLEKIVKNAVGYG-IKDKQKIVEGEINEVYDITPTKGDNLIVRVSREEH--NRFEAEKWALDNCRN-AGVSVPQIILLDEVET-DKEKLHICVENKIEGVSLQELIKQKVLSQSEIKDLTISAGEILSKIHSIKAKNFGEINSLGVGKFETWPEYMLEQNKNSDIVLEKAKNISIDPKLIQQAIEILEKTQDMYMNVDSRLAHSDYGIKHMLIHE-GKINGIIDFENCKSSDIAYDFSYWNYFGKNRPSVEWLMEGYKSNGNFG-ENFEARMHLVKIKIALSLLIYYSDAGHGFAKEITTNNLIEDITFFNYVK 5IGH Chain:A ((5-297)) ---------------------TTADTSQLYALAAR-HGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMVIQPGSSTPDWV-----VPQDSEVFAESFATALAALHAVPISAAVDAGML-IRTPTQARQKVADDVDR---VRREFV---VNDKRLHRWQRWL--DDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFG-EEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSGNEEYLAAAKAQL-----------

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1248 7682 6.16 26.86 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 6.16 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.370

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: