Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQYSERLGSINNAQFQKALDKFNLGLLTKTEKILFGNFGQNIFLTTTKG-NFVLRGKPHY--SWQFKNEKLMVDLLHEKTNTKVPHPYLIDENTDIFGWS----YVIMPRLGGRQLIDKNEQKNLTLKDWKEIASAYAINLIEMHKLTYKYSGKYTDNYIGIKPFSPSFSSWIIQSINILLDKSSSYNKKTTEQDKKWITELLNHRKEALEEPFKPTFTMQDYQITNTLVDKVDGQWIVTGVFDFMESYFGDCEVDLSRMFLKLHENDKELAYIFLNTYLRRKKIRRGFSDRFAIYIVLDRLLVWEWAQRTGKLWWDPDMSLSDWCKRYTKLDKNYLGL 5IGI Chain:A ((6-256)) --------TADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRL--PFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWV-----VPQDSEVFAESFATALAALHAVPISAAVDAGMLI----RTPTQARQKVADDVDRVRREFV-----VNDKRLHRWQRWLDDD---SSWPDFSVVVHGDLYVGHVLIDNTE---RVSGMIDWSEARVDDPAIDMAAHLMVFG---EEGLAKLLLTYEAAGG-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1024 -24444 -23.87 -100.59 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -23.87 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.316

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: