Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKHEKLKQLSPLLKQAISFQPMTSGFSGDRTFLVTTPSEKLVLKLSDIQK--YASLKKKTSILQNLKK-RGVLCSEVMEVGVSA---------EQNCTFRIFPFIEGENARASIRLLTEEEQYKIGRRAGRDLRIMHTCDAPTYVA------PWD-DRVLAKHER---YVYAYQSSGVAFSHDQFVLDFIQSHADVV--KERPNQFQHDDFHVGNIIIQRH---QYAGVIDFDQSDWGDPIHDFYKLSLFSRE--SSIPFSAGQLDAYLST-QTTHDFWLLFSIYTAMSCFSSIVWTLSYDPGHVKDMVNRVERMLTDHKRFTQLTPVWYTDWLDSCV 6EF6 Chain:A ((44-321)) ----------------ASLRLL-NLSEN-ATYLVEDGEHQSILRVHRQDYHQPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDPAD-VPRHVVHFEMVGGAEPDEES--LT----LDDFQTLGRITASLHEHSQRWTRPAGFGRFSWDWEHCLGDTPRWGRWQDAEGVGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHDPRLGEWQESWVAGYRSRRELPAADEAMLPSFVFLRRLLLLAWMGSH--------------------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1039 -8063 -7.76 -32.91 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -7.76 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.189

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: