Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRTVAAPAPALAEVACFSRDLCKE-LVKEWSSLDSSCFSISTVSGGITNMLLKVSVKEGSDSESSVTVRLYGPNTDLVIDRERELQGSGLGYLEYLRMEWFRHSSMLGHYHLQVYTQRLL--FCTHRCMETGEPAINASANNMKEPRIAAEIAKQLQKFHQVDIPGSKEPQ-LWNDIFKFLKKASVLKFEDNEKQKRYETISFREIQDEVKELKDLSDLLHAPVVFAHNDLLSGNLML-----NDLEEKLYFIDFEYGSYSYRGFDIANHFNE---------YAGFECDYNLYPDKDVQYHFFRNYLSDRPSELKNRLFASICEKRNVGYLAFVQYKVQEQDLDDLYTETNTFRLASHLYWALWALIQARVSPIDFDYLGYFFLRRHVVPNRKLYNL 3G15 Chain:B ((39-384)) ---------------------CKEFLP-AW-------------------MLFQC-----------VLLRL--------------------MVLESVMFAILAERS-LGPKLYGIFPQGRLEQFIPSRRLDTEELSL---------PDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFEN---LSTEEKSIIK--------------EEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDY----------------

General information: TITO was launched using: RESULT: Template: 3G15.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1097 -19116 -17.43 -72.41 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.72 3D Compatibility (PKB) : -17.43 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.304

(partial model without unconserved sides chains): PDB file : Tito_3G15.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3g15-query.scw PDB file : Tito_Scwrl_3G15.pdb: