Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFLTEANVLDYLTGRGFAGPEFVVRGEWTVRNLSRRNRNFRVTR-GGREFLVKQAGTWDLPGRASIEREADLCRRAATDPCFGALRPLVPDVYSYDPD-------HSILIFEFLPDEASLSDVPE-RLDARTARLAGELMADYHRQMQSAALA-EHFPGSL-PGYFSMHRWDSERLVTRSQGQRELVRLVKRHAAFAPALESAAAEWRPGALIHGDWKLENCLISNDGARMHVVDWELAGWGDARWDVATLLQSWWKRWVRDPAEYRLEAMRPELRALLNPSEDEVAAILRFAAVRMLQSAWESLQDIPAIHGEAVRMAQVSLNILTNPEWAGEAVFGHD 5IGI Chain:A ((26-251)) --------------------------PLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEV--SAKVEPEARVLAMLKNRL-----PFAVPDWRVANAELVAYPMLEDSTA-VIQPG-SSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVAD----DVDRVRREFV---VNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFG---------------EEGLAKLLLTY----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 856 -5755 -6.72 -26.89 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -6.72 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.274

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: