Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------MRQAIPWRDRSF--------------------RERFERWIEANVPNATWHVIKDTDLTYLLVGALNEERY---YAKAVTDISRGEAGLSAFLAERHPDFVPD-----VIAIDSARNWLLMRDIGGEALRERPDVRRYEAALRQYAQLQRQEIDQIETYLSYGIPDRRPATLKDEIQT------YLPELCAGLDRHQAEAMLALQDELLTMCDELA-TGMPMSLEHG-DLHGGNIFWRERTNDLCILDWGDATVTHPFFSVRVFWNALYDLLPEEDEVA--------WYKQIQTMRTVYLEAWSGVAPKD----------VLWRHLLI----AEELGCVYRALSWHVYVTRYRYDVAES---SDKPAQWLNFLLEYRDLKRRFP---------- 4FDT Chain:A ((21-425)) MQTKIQKYAGTAMPYPNRTDGMTPFYINHLGRHGARFPTSRKALDKVEKVLVSAQQENGLTSEGMALLSMIRRLSRLFDGQWGKLSKLGETEQEGIAGRMIRNYPQLFSNSAKIEAIATYVPRSINSMDAFLSCMIRHNPALQVQRSEGKQYNHILR-FFDLNKSYVNYKEKGDWLPIYKAFVHKKISPVPIMKKFLLNPEQYLDKEAEEFVMALFSVAAILPDTSIPLNLEDLFTLDEWHRYWQTQNLRQYMSK-SSAPVGK-MLPVAIAWPLLSEFIRSAQEVISGKSDYQANFRFAHAETVIPFVSLMGIEKTDVQVCRPDSVSVYWKDYEISPMAANVQWLFYRDRDQRIWVKILLNEEAAALPISTACFPYYSWEKTRIFFNQRIEMAKKTLSVFN

General information: TITO was launched using: RESULT: Template: 4FDT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1463 23427 16.01 73.44 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 16.01 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.237

(partial model without unconserved sides chains): PDB file : Tito_4FDT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4fdt-query.scw PDB file : Tito_Scwrl_4FDT.pdb: