Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKLKNLVLNTDQVANAILKNWNHD--ENSLNFWR-GSANYVYFFTLKGDRYWLRFSRKDEN-SLEQI-KAEIEFLLYLKENNFSAVYPIISNNHRYIEVVEDEGETYYAVVFNKAEGVNLEIEDMTEAEFECWGKSLASLHRLSKTFRPKEH--V---RNSWKQVLEVTSDILS--------DFP-NEQNAKDELKKVEKWLKSLPVTNENYGLIHYDFELDNVFFNKQ-SRLFKAIDFDSSMYHWYVMDIACTLGDLNEL---------DSPKAEDGLKHFLKGYRS--INGIEEEFIDLLPKFERFDNLISFAKLLRSLQDSSFPSEPEWLKRLRPRLLKKCDEYRKGFEKAW 4OCV Chain:A ((24-323)) ----------TNEALFDVASHFALEGTVDSIEPYGDGHINTTYLVTTDGPRYILQRMNTGIFPDTVNLMRNVELVTSTLKAQGKETLDIVRTTSGDTWAEI----DGGAWRVYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNFLSGFDANQLTETIAHFHDTPHRFEDFKKALAADELGRAAGCGPEIEFYLSHADQYAVVMDGLRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELRDSITAREAELLPFSGNLLTMEC------------------------------------------

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1133 29362 25.92 109.15 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 25.92 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.406

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: