Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence CTY---SVYDSIGKMVVEDKDLPTNRRDYYT---------IGKKK-----YTFYFKENCDFDYSKPPL----PRCWHVSGI 1SFU Chain:A ((6-75)) CTVNDAEIFSLVKKEV---LSLNTN--DYTTAISLSNRLKINKKKINQQLYKLQKEDTVKMVPSNPPKWFKNYNC------

General information: TITO was launched using: RESULT: Template: 1SFU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 127 17432 137.26 355.76 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 137.26 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.339

(partial model without unconserved sides chains): PDB file : Tito_1SFU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1sfu-query.scw PDB file : Tito_Scwrl_1SFU.pdb: