Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence YKCRISLMNGSDSTD--------DYADGKPGDTVNLEANNGDIVKVYIR---DNCYYDILNKEKM----PASYT--IKT---- 1SPK Chain:A ((1-72)) ---------GSSGSSGQKVKTIFPHTAGNNKTLLSFAQ--GDVLTLLIPEEKDGWLYGEHDTTKARGWFPSSYTKLLSGPSSG

General information: TITO was launched using: RESULT: Template: 1SPK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 129 -1557 -12.07 -30.53 target 2D structure prediction score : 0.90 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -12.07 2D Compatibility (Sec. Struct. Predict.) : 0.90 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.268

(partial model without unconserved sides chains): PDB file : Tito_1SPK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1spk-query.scw PDB file : Tito_Scwrl_1SPK.pdb: