Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPLRNSDRTREEILTRYFPQ-Y-----RLIAPQAP-SGLGGASCIIEQGE---------QRLVLRQNHDPLASSSHFRRQFRALKR-LPADLVPKPRFYRQGWMAVEYLAGEIGNTL-----PETAVLAAMLCHLHRQPRLGWRV--TLLPLLEQYWQQASADRR-------------TPFWLAQLKRLRKTGEPQPLRLSPLHMDVHAGNIVHTSL-------GARLIDWEYAGDGDVALELAAVWSEDD--------------------AA-RQRLITDYARVAHM------------NADALRRQVRRWRPWVVMLMAGWFEMRFQQSR-DKQFIALADDAWRQLQTKG 5FTG Chain:A ((4-371)) -PEPRTRRRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG------EAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDAYFH--

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 992 -16011 -16.14 -60.42 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -16.14 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.496

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ftg-query.scw PDB file : Tito_Scwrl_5FTG.pdb: