Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWQAISRLLSEQVGEGEIELRNELPGGEVHAAWHLRYA-GHDFFVKCDEREM-LRGFTAEADQLELLSRSKTVVVPKVWAVGSDR-DYS----FLVMDYLSPRPLDA--------HNAFILGQQLARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAAEKGITFGNIDAIVEHVQQRLAS---HQPQPSLLHGDLWSANCALGP-D-GPYIFDP-ACYWGDRECDLAMLPLH--TDQPPQIYDGYQSVSPLP-LD----FLDRQPIYQLYTLLNRARLFGGQHLATAQKAMDRLLAV 5IGH Chain:A ((10-299)) --SQLYALAAR-HGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISA-AVDA--GML--IRTPTQARQKVADDVDRVR-----REF---V----VNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSGNEEYLAAAKAQLAA----

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1142 -33721 -29.53 -128.71 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -29.53 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.352

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: