Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDAMTQGNFVYAFLKSCFTRLRGFAQFLLPFLRPKKRRGINYYN-PVELNKYAMQTLQKALKDDPGADIRDCLPLVY-NSAMRVISKTKARKEEELKRPPTPEFRTKINYVENAEIIFPLSDEVVSLLLQYSDK--DGELVGKQLVISLKQLIWESPKIWESAVRGVVLKCSDNVALKVIRQHPDYTEYTTLQYLAEKLPEIPVPSTYGVIRFK--PFTAYFMSYIPSMTLAEAWPSLNHEGKTSVQEQLEEIFSKLR-TLRKDDGYPLGGVGGEGVKEARVGNSAQEK-----TINTAASFVDLQFSLSEFLSKSYIQFIRTLQPPPPTGSVFTHGDVRKDNIMVDIGDNNTCTVTAI-------IDWEDAGFYPDYFECTTLTRTWLPHVED--DWYNYLPPCIDPARFPHHWLIDRLWGIHHPIF-------------- 4DGK Chain:A ((1-492)) MKPTTVIGAGFGGLALAIRLQAAGIPVLLLEQRYVYEDQGFTFDAGPTVI---TDPSAIEELFALAGKQLKEYVELLPVTPFYRLCWESGKVFNYD---------NDQTRLEAQIQQFNPRDVEGYRQFLDYSRAVFKEGYLGTVPFLSFRDMLRAAPQLAK-------LQAWRSVYSKVASYIEDEHLRQAFSFHSLLVGGNPTSSIYTLIHALEREWGVWFPR----------------GGTGALVQGMIKLFQDLGGEVVLNARVSHMETTGNKIEAVHLEDGRRFLTQAVASNADSNSLFVLYFGLNHHHDQLAHHTVCFGPRLAEDFSLYLHAPCVTDSSLAPEGCGSYYVLAPVPHLGTANLDWTVEGPKLRDRIFAYLEQHYMPGLRSQLVTHRMFTP-FDFRTITNLYLVGA--GIPGVIGSAKATAGLMLEDLI

General information: TITO was launched using: RESULT: Template: 4DGK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1903 -28577 -15.02 -77.23 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -15.02 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.177

(partial model without unconserved sides chains): PDB file : Tito_4DGK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4dgk-query.scw PDB file : Tito_Scwrl_4DGK.pdb: