Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDSFFDTPIPEDQVFSNAPKCRTRREATRHYLDHVHYTEPQPDVPGEILHHIVHPFSMGSITDRQITLQDNGTICKSTRIGRSNLGAEKHHLELVAMKTSVPVPRVHQYYVSEEFEHLVM--DNMPGTTLQQAWPTLSHLERKSIADQVVSLVQRLRELRSTHINAALLFRRPLRDGLRDATDLSMERINPYLSDEHIAAYINKRSAIMEGQLNVFTHGDLDWGNILIADKQVCGLIDLESSGFFP-----PYWEWIMVKRLSH--SLPKDSWFRLLEERLGAEDHSNWQGMWEVEQLIMALDEFSRWDLTPAGREMNRHRGWTEVVRILGPGVGDPPVVLYEMAAEHPWWLVSAADGEVKFTSRREWPTLMKSKTAV-- 5TC1 Chain:M ((1-393)) MRAFSTLDRENETFVYRSNWTPGRFNSTGAKTKQWHYPSP---------------YSRGALSVTSIFSLDARSCYSLFPVSQN--------------LTYIEVPQNVANRASTEVLQKVTQGNFNLGVALAEARSTASQ-----LATQTIALVKAYTAARRGNWRQALRYL-----ALNEDRKFRSKHVAGRWLE--LQFGWLPLMSDIQGAYEMLTKVHLQEFLPMRAVRQVGTNIKLDGQTTCNISRRIVIWFYINDARLAWLSSLGILNPLGIVWEKVPFSFVVDW--LLPVGNMLEGLTA-------PVGCSYMSGTVTDVITGESIIQGTAKAQISAMHRGVQSVWPTTGAYVKSPFSMVHTLDALALIRQRLSR

General information: TITO was launched using: RESULT: Template: 5TC1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain M - contact count / total energy / energy per contact / energy per residue : 1422 -11163 -7.85 -34.99 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain M : 0.66 3D Compatibility (PKB) : -7.85 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.199

(partial model without unconserved sides chains): PDB file : Tito_5TC1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5tc1-query.scw PDB file : Tito_Scwrl_5TC1.pdb: