Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAVFTALSATQLAPWLQRYPLG-QIVDLQGIVSGIDNTNYFLTTTRGAYVLTIFEHLS---ARAL-PFYLDFMRHLAARRMPVPVPVAQSDGALFSLLRGKPAALVSRLEGAVQMA--PAAHHCAEAGRMLARMHLAGKTFAAAAPQNP--RG----LAWQCEAARAIDP----FLR--PAQRRLLHHELAFQHSIQA-SA--EYARLPAGICHGDLFRDNVFFVTQQGDSAGHPCRLSGFFDFYFAGLDKWLFDIAVCINDWCVMPA-------SGQYEEARVRAFLTAYQA--VRAFTPLEAVYWPALLRAAALRFWLSRLHDFYVPRPAKILNVHDPAYFERILRTFIAKRL 4OCV Chain:A ((24-328)) -------TNEALFDVASHFALEGTVDSIEPYGDGHINTTYLVTTDGPRYILQRMNTGIFPDTVNLMRNVELVTSTLKAQGKETLDIVRTTSGDTWAEIDGGAWRVYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNFLSGFDAN-QLTETIAHFHDTPHRFEDFKKALAADELGRAAGCGPEIEFYLSHAD-Q--YAVVMDGLRDGSIPLRVTHNDTKLNNILMDA----TT---GKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELRDSITAREAELLPFSGNLLTMECGMRFL--------------------------------

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1182 -49087 -41.53 -179.15 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -41.53 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.318

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: