Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQYSERLGSINNAQFQKALDKFNLGLLTKTEKILFGNFGQNIFLTTTKG-NFVLRGKPHY--SWQFKNEKLMVDLLHEKTNTKVPHPYLIDENTDIFGWS----YVIMPRLGGRQLIDKNEQKNLTLKDWKEIASAYAINLIEMHKLTYKYSGKYTDNYIGIKPFSPSFSSWIIQSINILLDKSSSYNKKTTEQDKKWITELLNHRKEALEEPFKPTFTMQDYQITNTLVDKVDGQWIVTGVFDFMESYFGDCEVDLSRMFLKLHENDKELAYIFLNTYLRRKKIRRGFSDRFAIYIVLDRLLVWEWAQRTGKLWWDPDMSLSDWCKRYTKLDKNYLGL 5IGH Chain:A ((6-256)) --------TADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRL--PFAVPDWRVANAELVAYPMLEDSTAMVIQPGSSTPDWV-----VPQDSEVFAESFATALAALHAVPISAAVDAGMLI----RTPTQARQKVADDVDRVRREFV-----VNDKRLHRWQRWLDDD---SSWPDFSVVVHGDLYVGHVLIDNTE---RVSGMIDWSEARVDDPAIDMAAHLMVFG---EEGLAKLLLTYEAAGG-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 966 -29532 -30.57 -121.03 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -30.57 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.320

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: