Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASDRQDLSDRIARAIGHG-VRRLSPLSGGCVGDVLRAELEDGAAPVVVKVAD-D-AGGGLDIEGWMLRYLADTSRLPVPGVLHAEA---------GLLVMEEIQAGGGGI------SASCQEHAADLLADLHSVTWDAFGLERDTLIGGLHQPNPREASWRTFFRDHRLLYMADESHRAGRLPADVRRKVDTLAGRLDDWIEEPDAPALIHGDMWTGNVLCDG-GRIAGFIDPA-IYYGDAEIELAFSTLFG--TFGEPFFRRYQEKRPLR-PGFFEAR----RDLYNLYPLLVHVRLFGGSYVGSVSRTLDRFVG 5IGI Chain:A ((9-298)) ----TSQLYALAAR-HGLKLHGPLTVNELGLDYRIVIATVDDGR-RWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAG--MLI--RTPTQARQKVADDVD-----RVRR----EFVVN---DKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPR-LAHHIAERLAFGAVTYALFALDSGNEEYLAAAKAQLA----

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1185 -25212 -21.28 -96.23 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -21.28 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.214

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: