Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKLVKANLRRKEQAFIDVVSPLLQDVGINPSLVQGIDVKVGRNQSAVLEVDGNQQYFVKLIQGSDGVARFRRSKDFENWVINKSIKFATPKLLAFSKDQRALMFEFARCDTNLGEMLGDKFIDSVTAMKVAVAIGSLHTSTVSISDNIESDLPVLPPQFPGLISLDYFENATIGERNLWRILQNDAELLEALDLLVDSRINFVPIHGDFRPDQVFFRADEVVLLDFEEFRLGDPARDLGSFIGDLFYHKMSWIAVGAQGTDGRLTHESLLERGVDLLGQVRPIIQSFWEKYCIFCCGAQSETWKMTQPNLANRVTGYAGWHLFDRSLARSALSGRLTANDRALNGIGRNL-IINSGMFAQELGLGE 5IPX Chain:A ((32-322)) ---HLFNHLFR--YHYPS-WDQILQELDTLS---------VATLNPDCHVPALNVEKTLYLAKTIQILVQHRQSEPYLVPAARANLAYSLQQLYKLGN---------------------DKIRGVINGMLPLVDAGCI------------GFERELIKGLPRVLTLQYPHTAPCTEWCLSHFVGASGRLRSEVRDILTTH------NGTCAPSFEWMASVVKKFFLVETVIYEDFQD--TDFNVQLNLC-FFWTAVVQMYQRCIY-EQKLVHIISTSLTLLKSTARSFFAWYDLY------------RPNL-----GSAALVKYTEHLIRALTPD---CSDVELGELCSHLHHCKHALFS-------

General information: TITO was launched using: RESULT: Template: 5IPX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1152 -136896 -118.83 -487.17 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -118.83 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.222

(partial model without unconserved sides chains): PDB file : Tito_5IPX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ipx-query.scw PDB file : Tito_Scwrl_5IPX.pdb: