Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASAARPIRDYLPG--------LLVLAGGLYTV---------YYYYMYWIRVYGALCLPGYGDPVKVEVTQGIWIRLDAILFPTVF----GVILALL-----GVMLVW---KKKFGAAVVALVLSATVINWPAFIMI----AGGALLGLLFWRKTLLPTI-DLPDETMEWVDAEAGLSVLKSFRRLPSERLAYSFHNEGGGRFVVKETSSAESAERETRVLNAAAENGLPAPEAKATDGRFLLMPQL---DGFPFLQPLDFEAWTDEIAGILTTTHSAPKPDMKARRRPAEPVIPDRAFDRANWERAARIFRE----------ETADAVPVFVHGDFEPANVLFSGGRATGLTGWDHAGSGPAQLDVGRMRVALELIHGGNVADRFLAKYKNLSKDRSFRYSRYWDLLAVYGWLEQDPGSIASHWRRFGITTLSEDEVMRRLERFVKTLTDKE 2KR0 Chain:A ((1-407)) MTTSGALFPSLVPGSRGASNKYLVEFRAGKMSLKGTTVTPDKRKGLVYIQQTDDSLIHFCWKDRTSGNVEDDLIIFPDDCEFKRVPQCPSGRVYVLKFKAGSKRLFFWMQEPKTDQDEEHCRKVNEYLNNPPMPGALGASGSSGHELSALGGEGGLQSLLGNMSHSQLMQLIGPAGLGGLGGLGALTGPGLA-SLLGSSGPPGSSSSSSSRSQSAAVTPSSTTSSTRATPAPSAPAAASATSPSPAPSSGNGASTAASPTQPIQLSDLQSILATMNVPAGPAGGQQVDLASVLTPEIMAPILANADVQERLLPYLPSGESLPQTADEIQNTLTSPQFQQALGMFSAALASGQLG-------PLMCQFGLPAEAVEAANKGD-VEAFAKAMQNNAKP------------------EQKEGD--TKDKKDEEEDMSLD-----------------

General information: TITO was launched using: RESULT: Template: 2KR0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 930 -289 -0.31 -0.80 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -0.31 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.072

(partial model without unconserved sides chains): PDB file : Tito_2KR0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2kr0-query.scw PDB file : Tito_Scwrl_2KR0.pdb: