Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDIESILKNIIKIEPITTGKSNDKKYLLVLKNGTLLFERVGPISR-YDRFKMMYDELIDLKSLNEP---GVPIPYFV-TKED-DTAVLLTSYSKGIPLNEV-ITDKNCSLDRVKELGRKAGNLLASVHTLSPLVP-TD--SGDY---DVLFKNILSDYVNLS--LYLKHEQDMISFVLNHMKEVMTARPITYIHGNFDERILWVSPT-DRIFFVDTCDISSFDPFYDFKNIGVIT-RFENIEFAKSIIDSYTQGHPTDEFWLAFELYNAFKLMSTTIELIKEGKEKEAIDKFETVADDFHGFKEDEIGKPKWY 5IQC Chain:A ((27-287)) --------KVDSIEIIGSG-YDSVAYLVN----NEYIFKTKFSTNKKKGYAKEKAIYNFLN---TNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM--SEEEQNLLKRDIASFLRQMHGLDY-TDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNA---TTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNI-DIEKAKEYQDIVEEYYPIETIVYGIKNIK-----------------------------

General information: TITO was launched using: RESULT: Template: 5IQC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1067 18257 17.11 74.82 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 17.11 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.354

(partial model without unconserved sides chains): PDB file : Tito_5IQC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqc-query.scw PDB file : Tito_Scwrl_5IQC.pdb: