Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDAAIDGRAQSAEERMLRDALGEQLEGELRIISREPFGGGALTGFEELSPQARYWYVDTSGKRVEAETGFVLGDPGRPDARIWLHPADPRLPALAPASFPAAAATLMGRLGVSIDRPPELLVYRPGKRAMFRMRAGE-RETYLKIVRPSASGSIVELQESLRAGGVPVPHITGWSELGIVLTETADGVPLTSRLAELEPDRLLDSIDALRERIA---AVDT-----GRDARASLALRHDWYLARIDAALARAAE-GDPAVAPSAVLRDHLAAVTAVVTGADASAL-----VVDEAERRTIHGDLHVG----QLFVDADDASAIAGVIDID---------------TAGLGDPADDEA---------------ALIAHLIASIALARRDEGRSAG-FRRLLDAAAARWLAPGRPGRARVAHRTAVHVLAHALAPIERGDLETAEVELALGRDILRDADERPLI 4C2E Chain:A ((44-478)) ADSERDKAMDKIEKAYELISNEYVEK-----VDREKLLEGAIQGMLSTLNDPYSVYMD----KQTAKQFSDSLDSSFEGIGAEVGMEDGKIIIVSPFKKSPAEKAGLKPNDEIISINGESMAGKDLNHAVLKIRGKKGSSVSMKIQRPGTKK-----QLSFRIKRAEIPLETVFASEKKVQGHSVGYIAIST-FSEHTTEDFAKALRELEKKEIEGLVIDVRGNPGGYIQSVEEILKH--FVTKDQPYIQIAERNGDKKRYFSTLTHKKAYPVNVITDKGSAAASEILAGALKEAGHYDVVGDTSFGKGTVQQAVPMGDGSNIKLTLYKWLTPNGNWIHKKGIEPTIAIKQPDYFSAGPLQLKEPLKVDMNNEDVKHAQVLLKGLSFDPGREDGYFSKDMKKAVMAFQDQNKLNKTGIIDTRTAETLNQQIEKKKSDEKNDLQLQTALKS----------LF

General information: TITO was launched using: RESULT: Template: 4C2E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1884 51088 27.12 132.69 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 27.12 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.169

(partial model without unconserved sides chains): PDB file : Tito_4C2E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4c2e-query.scw PDB file : Tito_Scwrl_4C2E.pdb: