Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MESTDEPGDTVDTELFTRVVRHGLPDVTVETVTPGGPSWNPVTEVARVGFADRDPVYCKAAPGDHEGDLRAEAGTLDYVGATLRVRVPSVVRVTTEPVAALLTEPVAGRAVADEWFETTPERRATLAERLGRTLATVHTERFDRAGEITGGDPDGLALDHAPWPEVLRAGVTETQRRAPTDRFDPECARLLDAIDDSRDRLADPPARLLHRDPATPNCFDTGDDRLTLLDWGNSVVGDPVCDLVRAREQVLAPSREPAPDRLVAPLRRGYRAVAGGVPSAADREPVYEAAITLSTAGFVDRLAEWREESVAELTAWFRAELDGRLSAVE-- 3HAM Chain:A ((1-299)) -----------MVNLDAEIYEHLNKQIKINELRYLSSGDD--SDTFLCN---EQYVVKVPKRDSVRISQKREFELYRFLENC-KLSYQIPAVVYQSDRFNIMKYIKGERITYEQYHKLSEKEKDALAYDEATFLKELHS--IEIDCSVSLFSDALVNKKDKFLQDKKLLISILEKEQLLTDEMLEHIETIYENILNNAVLFKYTP-CLVHNDFSANNMIFRNNRLFGVIDFGDFNVGDPDNDFLCLLDCSTDDFGKEFGRKVLKYYQHK------------APEVAERKAELNDVYWSIDQIIYGYERKDREMLIKGVSELLQTQAEMFIF

General information: TITO was launched using: RESULT: Template: 3HAM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1321 -107767 -81.58 -362.85 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -81.58 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.258

(partial model without unconserved sides chains): PDB file : Tito_3HAM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ham-query.scw PDB file : Tito_Scwrl_3HAM.pdb: