Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------AAAGPTS--PKSGEQKQQVPVCLVQLLEGDLFWKFYTARPGELYAFAIENLSGIFITAKD---CTIKDVYGKKIRRRFTLKS--- 1F00 Chain:I ((753-841)) TLTIDDGNIEIVGTGVKGKLPTVWLQYGQVN---LKASGGNGKYTWRSANPA---IASVDASSG-QVTLKEKGTTTISVISSDNQTATYTIATPNS

General information: TITO was launched using: RESULT: Template: 1F00.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain I - contact count / total energy / energy per contact / energy per residue : 245 17109 69.83 244.41 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain I : 0.66 3D Compatibility (PKB) : 69.83 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.429

(partial model without unconserved sides chains): PDB file : Tito_1F00.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1f00-query.scw PDB file : Tito_Scwrl_1F00.pdb: