Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREPSSLQERDPDPFSEQMIALMVAAAGIECEVLDAEPLRTGSA-TTYRVDVRDQEGDRRTVVFKAAVEGD-ATGLRREPALLRYVADHTSIPVPDVYGSHLQPDD--LPAPSFVMEHVEGGSPTQASARENGDTGWPDGILDDIVRQAGRHLAALHDCGPVSGFGPITVDDGTLTVTDPASSWQSWLWELAAEIEGD-TRLDGLASTVQSVLDSEIPELPDVDPVLAHYDYQPRNVVVDEGAGRVRAVLDWGGARSVHDEFELAVTEQYCSGWAPLGSDKRQRVRASLYEGYRDRRSLATDEAFRRRRRLYLLLSTVASLSWAPYWSGPRAERFVASQRSLVESLLDQGP 5IGI Chain:A ((6-258)) -------------TADTSQLYALAAR-HGLKLHGPLTVNELGLDYRIVIATVD-DGR---RWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWV---------VPQDSEVFAESFATALAALHAV-PISAAVDAGML------IRTPTQARQKVADDVDRVRREFVVNDKRLHRWQRWLDDDSS--WPDFSVVVHGDLYVGHVLIDN-TERVSGMIDWSEARVDDPAIDMAAHLMVF----------GEEGLAKLLLTYEAAGGRV---------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1050 -16590 -15.80 -67.16 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -15.80 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.281

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: