Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHATSLPPELHQWVTRHLTGMDTAAVTDASWPRGASRVWRVRTDHGEVFVKLYP-STEKYEREVQGCEHATRA------LAADEAPRLLASDPDRPAVVLSALPGRVVRGLPLEQAEE-----QRVHRLAGGL-----------LRRWHDTPEPIGDRE-HQRIR-ASVTAQADEAAVILERLGDQLAPAERNLVRDAAHDLPDLARALPL--AYRHGDYSPRNWLWDEAAGHHSLIDFEESAPGLAVEDLVW-LCGAAWPTRP--DLRDAF-LTGYGRLLSSTEQRA--------LVLLTARLGVSYLSTGLTKADTVLIDRGRTVLQHMVRAPR-- 2Q83 Chain:A ((11-346)) --LSAEDAKKLTELAENVLQGWD---VQAEKIDVIQALVWKVHTDSGAVCLKRIHRPEKKALFSIFAQDYLAKKGMNVPGILPNKKGSLYSKHGSFLFVVYDWIEGRPFELTVKQDLEFIMKGLADFHTASVGYQPPNGVPIFTKLGRWPNHYTKRCK-QMETWKLMAEAEKEDPFSQLYLQEIDGFIEDGLRIKDRLLQSTYVPWTEQLKKSPNLCHQDYGTGNTLLGEN-EQIWVIDLDTVSFDLPIRDLRKMIIPLLDTTGVWDDETFNVMLNAYESRAPLTEEQKQVMFIDMLFPYELYDVIREKYVRKSALPKEELESAFEYERIKANALRQLI

General information: TITO was launched using: RESULT: Template: 2Q83.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1198 21382 17.85 73.48 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 17.85 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.316

(partial model without unconserved sides chains): PDB file : Tito_2Q83.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2q83-query.scw PDB file : Tito_Scwrl_2Q83.pdb: