Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLFENESMGFNHDEICATWNIPPLVSVQKPLTGTIHNVFLLTTR-EAKYVLRIYSYPP--EKRFRIVNEHAIARYVQSHHLPAIAPLPISSGCETFLERDGHFYALYPFAQGEQLSRAMLNSNQSHVGEIISAMGHSLAEVHLVLSSYCLPTTRPLSLVVNREQTIAKIEELEIVIPA-KEALDDLDQRVLSALRARKQYLLTASDVDVSELNALPWQPLHGDFQETNLFFSHGRVSAIIDWDQACSGPRAWEILRTLHYVFALDPSRCQRFLEAYQQVFPLPMEVLHLTAKVYTWVQLNNLWGWRSYYLDNNQSLRQLLSTSDSQPFTTRWAQLAKML 1ZYL Chain:A ((9-269)) ----QTLHPDTIMDALFEHGIRVDSGLTPL-NSYENRVYQFQDEDRRRFVVKFYRPERWTADQ--ILEEHQFALQLVNDEVPVAAPVAF-NGQ-TLLNHQGFYFAVFPSVGGRQFEA--DNIDQ------MEAVGRYLGRMHQTGRKQLFIHRP----TIGLNEYLIEPRKLFEDATLIPSGLKAAFLKATDELIAAV----TA-----HWREDFTVLRLHGDCHAGNILWRD--GPMFVDLDDARNGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPL----------------------------------------------

General information: TITO was launched using: RESULT: Template: 1ZYL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1119 -44717 -39.96 -174.00 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -39.96 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.249

(partial model without unconserved sides chains): PDB file : Tito_1ZYL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1zyl-query.scw PDB file : Tito_Scwrl_1ZYL.pdb: