Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTPSDRPLMAKLSAIAAYFALG--EIRSYKRAPGT-NHNYLVATEEGEYLFKIIVNTT---LEDV-LNGLPFLQRLQEQNFAATAYYLRSSSGQVFYHSPDCDAVVLQRLPGRNP-S--LSPAVCHEIGVVLARLHLVPCDNLPE-KRH-WLD---ARYLPETIEKAVNMY----G---TERLR---ETL-AVIDSLKHFQPATFPQCIIHGDLDTSNCLFEG--ERLVAFVDWQEVGVSAAMMDFVQTVIGFCFVEQDAGS-DYWALFNSELYHALYAGYTS--IRPLTPYEQAHLDAALKYVGLTQPAWSMLMWEHYHPGEQMIETNLLFWKYGLDKLTLPVL 4OCV Chain:A ((27-321)) -----------ALFDVASHFALEGTVDSIEPYGDGHINTTYLVTTDGPRYILQRMNTGIFPDTVNLMRNVELVTSTLKAQGKETL-DIVRTTSGDTWAEIDGGAWRVYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNFLSGFDANQLTETIAHFHDTPHRFEDFKKALAADELGRAAGCGPEIEFYLSHADQYAVVMDGLRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELRDSITAREAELLPFSGNLLTM---------------------------------------

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1098 -13310 -12.12 -50.42 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -12.12 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.308

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: