Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------MDSFFDTPIPEDQVFSNAPKCRTRREATRHYLDHVHYTEPQ--PDVPGEILHHIVHPFSMGSITDRQITLQDNGTICKSTRIGRSNLGAEKHHLELVAMKTSVPVPRVHQYYVSEEFEHLVMDNMPGTTLQQAWPTL---SHLERKSIADQVVSLVQRLRELRSTHINAALLFRRPLRDGLRDATDLSMERINPYL-------------SDEHIAAYINKRSAIMEGQL--NVFTHGDLDWGNILIADKQVCGLIDLESSGFFPPYWEWIMVKRLSHSLPKDSWFRLLEE--------RLGAEDHSNWQGMWE-------VEQLIMALDEFSRWDLTPAGREMNRHRGWTEVVRILGPGVGDPPVVLYEM----AAEHPWWLVSAADGEVKFTSRREWPTLMKSKTAV 4N0N Chain:A ((22-422)) SAVCTVCGAAPVAKSACGGWFCGNCVPYHAGHCHTTSLFANCGHDIMYRSTYCTMCEGSPKQMVPKVPHPILDHLLCHIDYGSKEELTLVVADGRT---TSPPGRYKVGH-----KVVAVVADVGGNIVFGCGPGSHIAVPLQDTLKGVVVNKALKNAAASEYVEGPPGSGKTFHLVKDVLAV----VGSATLVVPTHASMLDCINKLKQAGADPYFVVPKYTVLDFPRPGSGNITVRLPQVGTSEGETFVDEVAYFSPVDLARILTQGR-VKGYGDLNQLGCVGP-----------ASVPRNLWLRHFVSLEPLRVCHRFGAAVCDLIKGIYPYYEPAPHTTKVVFVPNPDFEKGVVITAYHKDRGLGHRTIDSIQGC---TFPVVTLRLPTPQSLTRPRAVVAVTRASQELYIYDPFDQLSGLLKF-

General information: TITO was launched using: RESULT: Template: 4N0N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1687 -1179 -0.70 -3.46 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -0.70 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.143

(partial model without unconserved sides chains): PDB file : Tito_4N0N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4n0n-query.scw PDB file : Tito_Scwrl_4N0N.pdb: