Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKQEIKLLDEAFALPENTKWSSFGEGHANETWLGEIDGDEPVVLRRHNPN-RTEEQILSEFKCLDLLHDLIGYEVPKALYGVNQERVTEV----EGAHYSLFELIEGETPPLD--KVETCKQSGQALANLHIKLWEERDAFKFAE-EERPGVSKET-LKSGAELLEALSSMEASKNIDGKYLTSEYWTDVEASLNDALGALAKLNETKHLIHGDFGPPNTLISENSG---ELVGILDWDECRWDLPVYDVAGVYPFLCE--IDKSLGEAFVDAYFAGLKGSSHPLAARENETRDLM---GAVQYVATYKEFELMVENHLDEPEYLAQLLEQLA 6EF6 Chain:A ((29-317)) ---TIAQQALTHYDVSDNASLRLL-NLSENATYLVEDG-EHQSILRVHRQDYHQPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDPADVPRHVVHFEMVGGAEPDEESLTLDDFQTLGRITASLHEHS---QR-WTRPAGFGRFSWDWEHCLGDTPRWGRW------QDAEGVGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHADLRLANLLVDS--STPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHDPRLGEWQESWVAGY-----RSRRELPAADEAMLPSFVFLRRLLLLAW--------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1223 -13843 -11.32 -51.46 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -11.32 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.318

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: