Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGWCADAPALIARLASQWELSL---GESLPDGASSIALRCRLPDGTPAVLKVSPDR---ALMTEQVDMLGWFAAS-GR-VPSVLAVDEE---S----GAMVLEEIVPGTPAGDM----PVAPLPGQWSDLLAALHGVAPPPLPTR-----VLRGRCEEAFARVGRRLAEPAISARMDVTAWDRAIQRCERLLNTEATTVLLHGDLHLGNVLDGGAQRVLMAIDPK-ACVGDPCFDAVDYVVAGAGLEGIETRCEQVAAACGLD----GDRLHAWSQVIAPFAAIAHLGSGGKEPLIDELLESAR 5IGH Chain:A ((9-285)) --------TSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMVIQ-PGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDR-VRR---EFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFGEEG-LAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSG--------------

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1017 -4035 -3.97 -16.27 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -3.97 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.328

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: