Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSAAEMGMAETADLRTRLAPLFPGREISLRSAAAPVASPLHRAVASACVLAQ-PDGADPLFLKLAHPDMQD-DLLDTDGAAARLAARAGVAPALVLA--RPGLLGFAYLALPWR-YARTGDLQPPATLAAVLAAKKRLHEGDNLGR-RFCPFTRIAQLQAQAEAAQVPLPAETAPLLRQAALIQQALIASGMDLRFCHNHAAASNIMLNG-SQMMLVDFDIGGDNDPWYDVGALLNEVCDFDAPRRAAIEAYAGRCDEALFARCRLYGAVDDLMWGLWGFTRAVTAPRDGIEFWKYGTWRLFQARTTMTMRDFELWLRTV 4R7B Chain:A ((37-304)) -----------------------EEVLSVEQ--------L-GGMTNQNYLAKTTN--KQYIVKFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYI-ES-AITLDST--SIKTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIP--YANYESVRNAVFSLEKRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTSQEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQG----EDFGDYGVNRYQRAVKGLASYG--------

General information: TITO was launched using: RESULT: Template: 4R7B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1236 -466 -0.38 -1.78 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -0.38 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.275

(partial model without unconserved sides chains): PDB file : Tito_4R7B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4r7b-query.scw PDB file : Tito_Scwrl_4R7B.pdb: