Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASDRQDLSDRIARAIGHG-VRRLSPLSGGCVGDVLRAELEDGAAPVVVKVAD-D-AGGGLDIEGWMLRYLADTSRLPVPGVLHAEA---------GLLVMEEIQAGGGGI------SASCQEHAADLLADLHSVTWDAFGLERDTLIGGLHQPNPREASWRTFFRDHRLLYMADESHRAGRLPADVRRKVDTLAGRLDDWIEEPDAPALIHGDMWTGNVLCDG-GRIAGFIDPA-IYYGDAEIELAFSTLFG--TFGEPFFRRYQEKRPLR-PGFFEAR----RDLYNLYPLLVHVRLFGGSYVGSVSRTLDRFVG 5IGH Chain:A ((9-298)) ----TSQLYALAAR-HGLKLHGPLTVNELGLDYRIVIATVDDGR-RWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAG--MLI--RTPTQARQKVADDVD-----RVRR----EFVVN---DKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPR-LAHHIAERLAFGAVTYALFALDSGNEEYLAAAKAQLA----

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1148 -27545 -23.99 -104.73 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -23.99 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.255

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: