Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKTEPSINLSLKRLVENPSYADACDILDERPLEFWLPTIEVILSQHQLAKKTVLRISNGSNALFDIDNEYILKLVPPNWDYQGISEVTSLTLLNLANHSENLSLAVPKMIGFGRVDNWFYVIMTKLKGLSMATVWPDLNTAQKLPLVKQLGQFMTELHQITSTQAFTPDPR-LSVDWPEYIKGLISDSVPRHARKGVSPELVTQISDYLQAADSDFNDIH--AVFIHMDLHPWNLMVEKHHGHYRLCGVVDFGDALVGNCQLLELATPIIFVCQGNKTLIDELVANYSPLNEVYLDRQVLQRQLMAVSLIRPACDFNFVLQQVPQSGKRDSWQAIAKQLFPLYKGRPA- 3HAM Chain:A ((1-299)) --------MVNLDAEIYEHLNKQI-KINELRYLSSGDDSDTFLCNE-----QYVVKVPKRDSVRISQKREFELYRFLEN---------------------CKLSYQIPAVVYQSD--RFNIMKYIKGERITYEQY-HKLSEKEKDALAYDEATFLKELHSIEIDCSVSLFSDALVNKKDKFLQDKKLLISILEKEQLLTDEMLEHIETIYENILNNAVLFKYTPCLVHNDFSANNMIFRNN----RLFGVIDFGDFNVGDPDNDFLCLLDCSTDDFGKEFGRKVLKYYQHKAPEVAERKAELNDVY---------WSIDQIIYGYERKDREMLIKGVSELLQTQAEMFIF

General information: TITO was launched using: RESULT: Template: 3HAM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1298 -67833 -52.26 -229.94 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -52.26 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.279

(partial model without unconserved sides chains): PDB file : Tito_3HAM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ham-query.scw PDB file : Tito_Scwrl_3HAM.pdb: