Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVELTDPDLERLRRRLAPA-HI-----EDLR--PLTGGASSLTYAGQRL--------GDRVVVKMAPPGIQ--PTRNRDVLRQCRIIKALRA-TPVPVPDVLWADAGDPPEIPPLFVMSWIDGSSLEPLFDLD------CACESEDIVAERFRSAAATMAQLHRIQPAAIGLGSEPIVGAEAEIDRWCRALETVD--PTLAAGWIDLGKALRAAAPP---PLPPAVVHGDFRLGNLRAVDGRITGVLDWEIWSVGDPRVDVGWFLINCDRQTYQRATPYAGAT---PSPAELVSIYCAAAGYRLPDLAWFQALACFKSAATWSLIVKHNRRRHRPDANVEAMAQVLPHLLSRACILLD 3ATT Chain:A ((4-328)) ---------AVISRWLSSVLPGGAAPEVTVESGVDSTGMSSETIILTARWQQDGRSIQQKLVARVAPAAEDVPVFPTYRLDHQFEVIRLVGELTDVPVPRVRWIETTGDVLGTPFFLMDYVEGVVPPDVMPYTFGDNWFAD-APAERQRQLQDATVAALATLHSIPNAQNTFSFLTDTTLHRHFNWVRSWYDFAVEGIGRSPLLERTFEWLQSHWPDDAAAREPVLLWGDARVGNVLYRDFQPVAVLDWEMVALGPRELDVAWMIFAHRVFQELAGLATLPGLPEVMREDDVRATYQALTGVELGDLHWFYVYSGVMWACVFMRTGARRV----------------------------

General information: TITO was launched using: RESULT: Template: 3ATT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1279 -49288 -38.54 -171.73 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -38.54 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.423

(partial model without unconserved sides chains): PDB file : Tito_3ATT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3att-query.scw PDB file : Tito_Scwrl_3ATT.pdb: