Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------CDFIIKQNGTQITSGNIWAGATASFLVSGKWAVVSATSECKLSLSGLPNNESYTILP-- 5H66 Chain:C ((9-75)) PIEARMNEIVHSLKSRGTRINFMDLFPYEQKEHLVVTFLAVLELMKNQLVLIEQEHNFSDIYITGSE

General information: TITO was launched using: RESULT: Template: 5H66.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 124 -3584 -28.90 -62.87 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain C : 0.66 3D Compatibility (PKB) : -28.90 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.169

(partial model without unconserved sides chains): PDB file : Tito_5H66.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5h66-query.scw PDB file : Tito_Scwrl_5H66.pdb: