Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence NFCAYYIKTLEGWELVGWVKIGGQDELTW-KGDSIGVKAE-DTDCKVVLVNGQ------------ 1GCQ Chain:A ((2-57)) ---STYVQALFDFD------PQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPV

General information: TITO was launched using: RESULT: Template: 1GCQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 115 -9247 -80.40 -220.15 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -80.40 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.478

(partial model without unconserved sides chains): PDB file : Tito_1GCQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1gcq-query.scw PDB file : Tito_Scwrl_1GCQ.pdb: