Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------------------------------------------------------------------------------------------------AAAFGPLTRI-D-KPFTQYRITYTAPNKKP--VVNKLGYGNTAKLS--VDGITYTAVADAKCKFVIVGSPWPAGHFTVGQV-------------------------------------------- 2JGR Chain:A ((4-299)) FPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKGDAARYVEEARKFGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATSVGIPEALDKALKLAIAGDAIAIDMAQVNKQTCFINMATGGFGTIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALINDGLLQLRIFTGDNPNIIEGASSWFDIQAPHDITFNLDGEPLSGQNFHIEILPAALRCRLPPDCPLLR

General information: TITO was launched using: RESULT: Template: 2JGR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 274 -11920 -43.50 -158.93 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -43.50 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.353

(partial model without unconserved sides chains): PDB file : Tito_2JGR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2jgr-query.scw PDB file : Tito_Scwrl_2JGR.pdb: