Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPFRSNNPITRDELLSRFFPQY-------HPVTTFNSGLSGGSFLIEHQG----------QRFVVRQPHDPDAPQSAFLRQYRALSQLP-ACIAPKPHLYL-----RDWMVVDYLPGAVKTYLPDTNELAGLLYYLHQQPRFGWR---I----TLLPLLELYWQQSDPAR-------RT----VGWLRMLKRLRKAR--EPRPLRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVEN---------------------TEQHRQLVNDYATRAK----IY---PAQLWRQVRRWFPWLLMLKAGWFEYRWRQT---GDQQFIRLADDTWRQLLIKQ 3C5I Chain:D ((14-352)) -----PLYIK--KICLEKVPEWNHFTEDNLRVKQIL--LTNQLFEVGLKEETANNYNSIRTRVLFRIYGKHVDELYNTISEFEVYKTMSKYKIAPQLLNTFNGGRIEEWLYGDPLRIDDLKNPTILIGIANVLGKFHTLSRKRHLPEHWDRTPCIFKMMEKWKNQLFKYKNIEKYNCDIHKYIKESDKFIKFMKVYSKSDNLANTIVFCHNDLQENNIINTNKCLRLIDFEYSGFNFLATDIANFFIETSIDYSVSSYPFFEIDKKKYISYENRKLFITAYLSNYLDKVVPTPKLIDEILEAVEVQALGAHLLWGFWSIIRGYQTKSYNEFDFFLYAEQRLKMY----

General information: TITO was launched using: RESULT: Template: 3C5I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 993 -23014 -23.18 -88.17 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain D : 0.65 3D Compatibility (PKB) : -23.18 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.371

(partial model without unconserved sides chains): PDB file : Tito_3C5I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3c5i-query.scw PDB file : Tito_Scwrl_3C5I.pdb: