Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAHVPSGPDELTPEWLSRALGAEVTSVSWERVGSGQIGACYRLTLKGEEKVPARLVAKMASEDPTTRAFLASAYRTEVLFYRDVVPTVAVRTPRCHYQAIAD---------DNSAFVLLLEDMAPAEQGDQLAGCTPQQAAAAVTNLAGLHGPRWCDKTLTEIPWLSVVTEDDAAVLTEVFAPAVDAFVQRFTGRLSDEDIDTLRRVADRIAAWAV---ARPERFGLVHGDYRLDNLLFAPPPQ-QTVTAVDWQTLSIGHPVRDLSFFLGTAMDPAERSAHERALVETYHTALTGYGVDDYDLQSCWEDYRFGALQGPLITVLGCAVGTPTERGDRMFLAMTRRSCAAIRELGSLELI 6EF6 Chain:A ((50-292)) ----------------------------------NLSENATYLVEDG-----EHQSILRVHRQDYH----QPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDPAD-VPRHVVHFEMVGGAEPDEE--SLTLDDFQTLGRITASLHEHSQRWTRPA---GFGRFSWDWEHCL-GDT-PRWGRWQDAEG--VGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHDPRLGEWQESWVAGYRSR------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 912 48 0.05 0.21 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 0.05 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.156

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: