Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQATLDWLAGLARQHPEVFGGHPGEMAVLEASRSSSHESRVYGSGGALVHVKFFRRTLGRTSVTYDPCREMKAEYDTLREFEKNGFASGRHRVVRTLGINESLDCALATAYVPGDTLLSIIQRTLKGGDDGELLEALGLAAGFLRKVHGLMPQSPKVD-AAETFYSYMKPLF-------YLEEQGALDGFHRRVTKGL-SRWYGHRPLFEQRGVTVHGDANPSNFKIHEG-VIYGLDLERSRPGRSPLLDVGTMVAELEHHCAYVAGDAGRAKPYVERFLRAYAPSPADRKKIDELLPFYVSRGLLKIAMLGYWKHGYRRSLVEAGARRIEEGP 4DCA Chain:A ((41-270)) --------------------------ELRYLS--SGDDSDTFLC-NEQYVVKVPKRDS--------VRISQKRELELYRFLENCKL---SYQIPAVVYQS---DRFNIMKYIKGERITYEQYHKL---SEKEKDALAYDEATFLKELHSIEIDCSVSLFSDALVNKKDKFLQDKKLLISILEKEQLLTDEMLEHIETIYENILSNAVLFKYTPCLVHNDFSANNMIFRNNRLFGVIDFGDFNVG-DPDNDFLCLLDCSTD---------DFGKEFGRKVLKYYQHK------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4DCA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 828 -19737 -23.84 -89.71 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -23.84 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.268

(partial model without unconserved sides chains): PDB file : Tito_4DCA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4dca-query.scw PDB file : Tito_Scwrl_4DCA.pdb: