Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGVKTKLFYEDIKPFFDI----KSLQETKNGESHTVYILDN----DYILKIYEEE-NLFNIDAEIELLNYTKK---LCVPKVIKDDIFIKGKRGLVFSKAKGESVVEF-V-----------KSTHLEQIGQFLNSFHKMTKNKKHD-NLS----AFGKSQLKVMIEKTCN--KAFKDE----FDCLK-I--ELKNDGIIHGDLFLDNATFCGDKLTCVFDFSDACEGDFIFDLAVVALSWCSNKEEINILLKAYDKEIKLDDFIIYLRYASLYYCVKRHLTNRDYDNIIFKNKFIDLI 5UXD Chain:A ((13-254)) -----------LAARHGLDLDGGTLRTEEIGLDFRVAFARAHDGGDWVLRLPRRPDVLERAAVEGRLLAMLAPHLDVAVPDWRIS-----TSELIAYPLLPGSPGLTVAADGEVSWHVDMASTVYARSLGSVVAQLHAVDAEAAAATGIEVRSPAQVRGAWRQDLARVGAEFEIAPALRERWEAWLADDGCWPGHSVLTHGELYPAHTLVEDERITAVLDWTTAAVGDPAKDLMFHQVSA--PSAIFEVALQAYAEGGGR--------------------------------------

General information: TITO was launched using: RESULT: Template: 5UXD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 693 -6754 -9.75 -33.11 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -9.75 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.347

(partial model without unconserved sides chains): PDB file : Tito_5UXD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxd-query.scw PDB file : Tito_Scwrl_5UXD.pdb: