Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGWCADAPALIARLASQWELSL---GESLPDGASSIALRCRLPDGTPAVLKVSPDR---ALMTEQVDMLGWFAAS-GR-VPSVLAVDEE---S----GAMVLEEIVPGTPAGDM----PVAPLPGQWSDLLAALHGVAPPPLPTR-----VLRGRCEEAFARVGRRLAEPAISARMDVTAWDRAIQRCERLLNTEATTVLLHGDLHLGNVLDGGAQRVLMAIDPK-ACVGDPCFDAVDYVVAGAGLEGIETRCEQVAAACGLD----GDRLHAWSQVIAPFAAIAHLGSGGKEPLIDELLESAR 5IGI Chain:A ((9-285)) --------TSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA-VIQ-PGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDR-VRR---EFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFGEEG-LAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSG--------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1057 -1024 -0.97 -4.14 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -0.97 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.317

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: